Resistin Antibody [1.2_3D7-1D11]

Primary Antibodies

Resistin lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚ C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚ C when reconstituted.

Resistin is a cytokine that is specifically secreted by adipocytes and seems to suppress the ability of insulin to stimulate glucose uptake into adipose cells. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes.

None

This IgG2bK antibody was purified from ascites fluid by Protein A affinity chromatography.

ELISA:
In a sandwich ELISA (assuming 100 μ L/well), a concentration of 4.0-8.0 μ g/mL of this antibody will detect at least 200 pg/mL of recombinant human Resistin when used with our biotinylated antigen affinity purified anti-human Resistin as the detection antibody at a concentration of at least 0.5-1.0 μ g/mL.
Western Blot:
To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.

Monoclonal

Homo sapiens

Centrifuge vial prior to opening.