FGF-17 Antibody

Primary Antibodies, Functional Antibodies

Room Temp

Stem Cell, Functional Assays

None

Human FGF-17 specific antibody was purified by affinity chromatography employing immobilized hFGF-17 matrix.

ELISA:
Sandwich:
To detect FGF-17 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Polyclonal Biotinylated Anti-Human FGF-17 (38-295) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-17.
Western Blot:
: To detect hFGF-17 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant FGF-17 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of FGF-17 (200.0 ng/mL), a concentration of 2.1 – 3.2 μ g/mL of this antibody is required.

Polyclonal

Homo sapiens

Centrifuge vial prior to opening.