Adiponectin Antibody [1.3-13A05-2D09]

Primary Antibodies

The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-80˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.

None

This antibody was purified from cell culture by Protein A affinity chromatography.

ELISA:
Sandwich:
In a sandwich ELISA (assuming 100μ L/well), a concentration of 2.0-4.0 μ g/mL of this antibody will detect at least 500 pg/mL of recombinant Human Adiponectin when used in conjunction with compatible detection reagents at a concentration of approximately 0.5-1.0 μ g/mL.
Western Blot:
To detect Human Adiponectin by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μ g/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Adiponectin is 0.5-1.0 ng/lane, under reducing conditions and 1.0-2.0 ng/lane, under non-reducing conditions.

Monoclonal

Homo sapiens

Centrifuge vial prior to opening.