IL-10 Antibody [15B9-1-2]

Primary Antibodies, Functional Antibodies

Room Temp

Infectious Disease, Neuroscience, Cancer, Stem Cell, Chemokines & Cytokines, Functional Assays

None

This IgG2A antibody was purified from cell culture by Protein G affinity chromatography.

ELISA:
Sandwich:
In a sandwich ELISA (assuming 100μ L/well), a concentration of 4.0-8.0 μ g/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with ProSci’s Polyclonal biotinylated antigen affinity purified anti-murine IL-10 as the detection antibody at a concentration of approximately 0.5-1.0 μ g/mL.
Western Blot:
To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/mL), a concentration of 3.0-5.0 μ g/mL of this antibody is required.

Monoclonal

Homo sapiens

Centrifuge vial prior to opening.