Vergleich

FOXP3 Antibody

ArtNr PRS-45-055-0.1mg
Hersteller ProSci
Menge 0.1 mg
Kategorie
Typ Antibody Primary
Format Liquid
Applikationen WB, FC, IF, ICC, ELISA
Specific against Human (Homo sapiens)
Host Caprine
Konjugat/Tag Unconjugated
Citations Chatila TA, Blaeser F, Ho N, Lederman HM, Voulgaropoulos C, Helms C, Bowcock AM. JM2, encoding a fork head-related protein, is mutated in X-linked autoimmunity-allergic disregulation syndrome. J Clin Invest. 2000 Dec;106(12):R75-81.
NCBI FOXP3
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias FOXP3,SCURFIN,forkhead box P3,JM2,AIID,IPEX,PIDX,XPID,DIETER,JM2 protein,immunodeficiency,polyendocrinopathy,enteropathy,X-linked,immune dysregulation,polyendocrinopathy,enteropathy,X-linked,MGC141961,MGC141963,FOXP3delta7,scurfin
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and store at -20˚ C. Minimize freezing and thawing.
Manufacturer - Research Area
Signal Transduction
By Type
Signal Transduction
Modifications
None
Protein Gi #
31982943
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Positive Control 1
Positive Control 2
Cat. No. 11-533 - Human Tonsil Tissue Slide
Concentration
500 ug/mL
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Application Note
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 48-50kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2kDa according to NP_054728.2). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate.Immunofluorescence: Strong expression of the protein seen in the nucleoplasm of U2OS cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.


Immunocytochemistry: CD25-sorted (Treg) Human blood cells with nuclear speckled staining. Recommended concentration 2-4ug/ml for overnight staining.

Manufacturer - Specificity
This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)
Immunogen
The immunogen for this antibody is: SQRPSRCSNPTPGP
Clonality
Polyclonal
NCBI Official Name
forkhead box P3
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 0.1 mg
Lieferbar: In stock
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