FOXP3 Antibody

ArtNr	PRS-45-055-0.1mg
Hersteller	ProSci
Menge	0.1 mg
Kategorie	Antibodies Immunohistochemistry Western Blot ELISA Immunocytochemistry Primary Antibodies CHiP
Typ	Antibody Primary
Format	Liquid
Applikationen	WB, FC, IF, ICC, ELISA
Specific against	Human (Homo sapiens)
Host	Caprine
Konjugat/Tag	Unconjugated
Citations	Chatila TA, Blaeser F, Ho N, Lederman HM, Voulgaropoulos C, Helms C, Bowcock AM. JM2, encoding a fork head-related protein, is mutated in X-linked autoimmunity-allergic disregulation syndrome. J Clin Invest. 2000 Dec;106(12):R75-81.
NCBI	FOXP3
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	FOXP3, SCURFIN, forkhead box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2 protein, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, MGC141961, MGC141963, FOXP3delta7, scurfin
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Manufacturer - Applications	Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 48-50kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2kDa according to NP_054728.2). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate.Immunofluorescence: Strong expression of the protein seen in the nucleoplasm of U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml. Immunocytochemistry: CD25-sorted (Treg) Human blood cells with nuclear speckled staining. Recommended concentration 2-4ug/ml for overnight staining.
Shipping Temperature	blue ice or room temperature
Storage Conditions	Aliquot and store at -20°C. Minimize freezing and thawing.
Manufacturer - Research Area	Signal Transduction
Protein Gi #	31982943
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Positive Control 1	PRS-1315-0.1mg
Concentration	500 ug/mL
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Immunogen	The immunogen for this antibody is: SQRPSRCSNPTPGP
Clonality	Polyclonal
NCBI Official Name	forkhead box P3
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

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Menge: 0.1 mg

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