Hersteller |
ProSci
|
Kategorie |
|
Typ |
Antibody Primary |
Specific against |
Human |
Format |
Liquid |
Applikationen |
WB, ELISA |
Menge |
0.1 mg |
Host |
Goat |
ArtNr |
PRS-45-159-0.1mg |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Signal Transduction |
Homology |
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow |
Immunogen |
The immunogen for this antibody is: ASRIGLRMQLMRE-C |
Applications |
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 55kDa band observed in Human Thymus and in lysates of cell line Jurkat and approx. 60kDa in lysates of cell line HeLa, (calculated MW of 52.9kDa according to NP_009093.1 and 54.2kDa according to NP_001161299.2). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on cell line NIH3T3 for this particular batch. |
Specificity |
. |
Positive Control 1 |
Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 45-159P - TFEB Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
TFEB |
Accession # |
NP_009093.1, NP_001161299.2, NP_001258872.1 |
Protein Gi # |
24307933 |
Ncbi Gene Id # |
7942 |
Ncbi Official Symbol |
TFEB |
Ncbi Official Full Name |
transcription factor EB |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P19484 |
Background References 1 |
Steingrimsson E, Sawadogo M, Gilbert DJ, Zervos AS, Brent R, Blanar MA, Fisher DE, Copeland NG, Jenkins NA. Murine chromosomal location of five bHLH-Zip transcription factor genes. Genomics. 1995 Jul 20; 28(2):179-83. |
1st Image Caption |
45-159 (1.0ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence. |
2nd Image Caption |
45-159 (0.5ug/ml) staining of HeLa (A) and (0.3ug/ml) Jurkat (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.