Hersteller |
ProSci
|
Kategorie |
|
Typ |
Antibody Primary |
Specific against |
Human |
Format |
Liquid |
Applikationen |
FC, IF, IHC, ELISA |
Menge |
0.1 mg |
Host |
Goat |
ArtNr |
PRS-45-162-0.1mg |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Signal Transduction , Cancer |
Homology |
Expected Species Reactivity based on sequence homology: Mouse |
Immunogen |
The immunogen for this antibody is: C-SSQELSGGPGDGP |
Applications |
Peptide ELISA: antibody detection limit dilution 1:2000. Immunohistochemistry:In paraffin embedded Human Breast shows nuclear staining in epithelial duct cells. Recommended concentration: 2-4ug/ml. Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. |
Specificity |
. |
Positive Control 1 |
Cat. No. 1211 - HepG2 Cell Lysate |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 45-162P - TRIM28 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
TRIM28 |
Accession # |
NP_005753.1 |
Protein Gi # |
5032179 |
Ncbi Gene Id # |
10155 |
Ncbi Official Symbol |
TRIM28 |
Ncbi Official Full Name |
tripartite motif containing 28 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q13263 |
Background References 1 |
Friedman JR, Fredericks WJ, Jensen DE, Speicher DW, Huang XP, Neilson EG, Rauscher FJ 3rd. KAP-1, a novel corepressor for the highly conserved KRAB repression domain. Genes Dev. 1996 Aug 15; 10(16):2067-78. |
1st Image Caption |
45-162 (2ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. |
2nd Image Caption |
45-162 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue) |
3rd Image Caption |
45-162 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue) |
4th Image Caption |
45-162 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) fol |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.