AIRE Antibody

ArtNr	PRS-45-225-0.1mg
Hersteller	ProSci
Menge	0.1 mg
Kategorie	Neuroscience Targets for Rodent Neurosciences Antibodies Immunohistochemistry Western Blot ELISA Primary Antibodies CHiP
Typ	Antibody Primary
Format	Liquid
Applikationen	WB, FC, IF, IHC, ELISA
Specific against	Human (Homo sapiens)
Host	Caprine
Konjugat/Tag	Unconjugated
Citations	Kumar PG, Laloraya M, Wang CY, Ruan QG, Davoodi-Semiromi A, Kao KJ, She JX. The autoimmune regulator (AIRE) is a DNA-binding protein. J Biol Chem. 2001 Nov 2;276(44):41357-64. Epub 2001 Aug 31.
NCBI	AIRE
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	AIRE, autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy), APS1, APSI, PGA1, APECED, AIRE protein, autoimmune regulator (APECED protein), autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy), autoimmune regulator, AIRE1, OTTHUMP00000109529, autoimmune polyendocrinopathy candidiasis ectodermal dystrophy
Lieferbar
Manufacturer - Applications	Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary testing showed an approx 60kDa band in nuclear lysates of cell line Jurkat and in Human Spleen lysates (calculated MW of 57.7kDa according to NP_000374.1). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Smooth Muscle lysate. This product has been successfully used in Western blot by an anonymous customer on HEK293 cell lysate.Immunohistochemistry:In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration: 2.5ug/ml.Immunofluorescence: Strong expression of the protein seen in the nucleoplasm and nuclear membranes of U2OS and HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
Shipping Temperature	blue ice or room temperature
Storage Conditions	Aliquot and store at -20°C. Minimize freezing and thawing.
Manufacturer - Research Area	Cancer
Protein Gi #	4557291
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Positive Control 1	PRS-1210-0.1mg
Concentration	500 ug/mL
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Immunogen	The immunogen for this antibody is: C-QSMARPAAPFPS
Clonality	Polyclonal
NCBI Official Name	autoimmune regulator
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 0.1 mg

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