AIRE Antibody

Cancer

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary testing showed an approx 60kDa band in nuclear lysates of cell line Jurkat and in Human Spleen lysates (calculated MW of 57.7kDa according to NP_000374.1). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Smooth Muscle lysate. This product has been successfully used in Western blot by an anonymous customer on HEK293 cell lysate.Immunohistochemistry:In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration: 2.5ug/ml.Immunofluorescence: Strong expression of the protein seen in the nucleoplasm and nuclear membranes of U2OS and HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Cat. No. 1210 - 293 Cell Lysate

Polyclonal

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.

Aliquot and store at -20˚ C. Minimize freezing and thawing.

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

AIRE

4557291

AIRE

Homo sapiens

Kumar PG, Laloraya M, Wang CY, Ruan QG, Davoodi-Semiromi A, Kao KJ, She JX. The autoimmune regulator (AIRE) is a DNA-binding protein. J Biol Chem. 2001 Nov 2; 276(44):41357-64. Epub 2001 Aug 31.

45-225 (2ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

45-225 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nucleoplasm and nuclear membrane staining. Actin f