Hersteller |
ProSci
|
Kategorie |
|
Typ |
Antibody Primary |
Specific against |
Human |
Format |
Liquid |
Applikationen |
WB, IHC, ELISA |
Menge |
0.1 mg |
Host |
Goat |
ArtNr |
PRS-45-732-0.1mg |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer |
Immunogen |
The immunogen for this antibody is: QQTSVRREDKCETD |
Applications |
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 55kDa band observed in Human Heart lysates (calculated MW of 55.8kDa according to NP_000852.1). Recommended concentration: 0.5-1.5ug/ml.Immunohistochemistry:In paraffin embedded Human Lung shows membranous staining of epitheilial cells in tertiar bronchus and in alveoli. Recommended concentration, 2-4ug/ml. |
Specificity |
. |
Positive Control 1 |
Cat. No. 1301 - Human Heart Tissue Lysate |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 45-732P - Histamine Receptor H1 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
HRH1 |
Accession # |
NP_000852.1 |
Protein Gi # |
4504491 |
Ncbi Gene Id # |
3269 |
Ncbi Official Symbol |
HRH1 |
Ncbi Official Full Name |
histamine receptor H1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P35367 |
Background References 1 |
Bryce PJ, Mathias CB, Harrison KL, Watanabe T, Geha RS, Oettgen HC. The H1 histamine receptor regulates allergic lung responses. J Clin Invest. 2006 Jun; 116(6):1624-32. Epub 2006 May 4. |
1st Image Caption |
45-732 (0.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
2nd Image Caption |
45-732 (4ug/ml) staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.