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NRAS Antibody

NRAS Antibody

Hersteller	ProSci
Kategorie	Oncology Antibodies Immunohistochemistry Western Blot ELISA Primary Antibodies By Organ Brain Cancer Nervous System Cancer
Typ	Antibody Primary
Specific against	Human, Rat
Format	Liquid
Applikationen	WB, IHC, ELISA
Menge	0.1 mg
Host	Goat
ArtNr	PRS-45-982-0.1mg
eClass 6.1	32160702
eClass 9.0	32160702
Lieferbar
Shipping	blue ice or RT
By Research Area	Cancer
Homology	Expected Species Reactivity based on sequence homology: Mouse, Dog
Immunogen	The immunogen for this antibody is: C-RMKKLNSSDDGTQ
Applications	Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 21kDa band observed in Human lysates of cell line A431 and of Rat Intestines (calculated MW of 21.2kDa according to NP_002515.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH3T3 for this particular batch.Immunohistochemistry:In paraffin embedded Human Small Intestine shows staining of cytoplasm and membrane of enterocytes. Recommended concentration: 2-3ug/ml.
Specificity	.
Positive Control 1	Cat. No. 1202 - A431 Cell Lysate
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products	Cat. No. 45-982P - NRAS Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	NRAS
Accession #	NP_002515.1
Protein Gi #	4505451
Ncbi Gene Id #	4893
Ncbi Official Symbol	NRAS
Ncbi Official Full Name	neuroblastoma RAS viral (v-ras) oncogene homolog
Ncbi Organism	Homo sapiens
Swissprot #	P01111
Background References 1	Thomas NE, Edmiston SN, Alexander A, Millikan RC, Groben PA, Hao H, Tolbert D, Berwick M, Busam K, Begg CB, Mattingly D, Ollila DW, Tse CK, Hummer A, Lee-Taylor J, Conway K. Number of nevi and early-life ambient UV exposure are associated with BRAF-mutant melanoma. Cancer Epidemiol Biomarkers Prev. 2007 May; 16(5):991-7.
1st Image Caption	45-982 (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
2nd Image Caption	45-982 (0.01ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
3rd Image Caption	45-982 (0.1ug/ml) staining of Rat Intestines lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 0.1 mg

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