RAD17 Antibody

For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

blue ice or room temperature

77 kDa

The protein encoded by this gene is highly similar to thegene product of Schizosaccharomyces pombe rad17, a cell cyclecheckpoint gene required for cell cycle arrest and DNA damagerepair in response to DNA damage. This protein shares strongsimilarity with DNA replication factor C (RFC), and can form acomplex with RFCs. This protein binds to chromatin prior to DNAdamage and is phosphorylated by the checkpoint kinase ATR followingdamage. This protein recruits the RAD1-RAD9-HUS1 checkpoint proteincomplex onto chromatin after DNA damage, which may be required forits phosphorylation. The phosphorylation of this protein isrequired for the DNA-damage-induced cell cycle G2 arrest, and isthought to be a critical early event during checkpoint signaling inDNA-damaged cells. Eight alternatively spliced transcript variantsof this gene, which encode four distinct proteins, have beenreported. Two pseudogenes, located on chromosomes 7 and 13, havebeen identified.

Batch dependent

This RAD17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human RAD17.

Cell cycle checkpoint protein RAD17

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.