Cyclin D1 Antibody

For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:2000

For IF starting dilution is: 1:10~50

blue ice or room temperature

34 kDa

CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. It has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of the gene encoding this protein, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.

This antibody is purified through a protein A column, followed by peptide affinity purification.

Supplied in PBS with 0.09% (W/V) sodium azide.

This Cyclin D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from human Cyclin D1.

G1/S-specific cyclin-D1

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.