Hersteller |
ProSci
|
Kategorie |
|
Typ |
Antibody Primary |
Specific against |
Human |
Format |
Liquid |
Applikationen |
WB, FC, IF, IHC-P |
Menge |
200 ul |
Host |
Rabbit |
ArtNr |
PRS-63-088-200ul |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Cell Cycle, Neuroscience, Signal Transduction |
Immunogen |
This FGFR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-55 amino acids from the N-terminal region of human FGFR4. |
Applications |
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:200
For IHC-P starting dilution is: 1:50~100 |
Predicted Molecular Weight |
88 kDa |
Purification |
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
FGFR4 |
Accession # |
P22455 |
Protein Gi # |
13432140 |
Ncbi Gene Id # |
2264 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
FGFR4 |
Ncbi Official Full Name |
Fibroblast growth factor receptor 4 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P22455 |
Background |
FGFR4 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. The genomic organization of the gene, compared to members 1-3, encompasses 18 exons rather than 19 or 20. Although alternative splicing has been observed, there is no evidence that the C-terminal half of the IgIII domain of this protein varies between three alternate forms, as indicated for members 1-3. This particular family member preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis. |
Background References 1 |
References for protein: |
Background References 2 |
Jezequel, P., et al., Br. J. Cancer 90(1):189-193 (2004). |
Background References 3 |
Qian, Z.R., et al., J. Clin. Endocrinol. Metab. 89(4):1904-1911 (2004). |
Background References 4 |
Yu, S., et al., J. Biol. Chem. 278(22):19597-19602 (2003). |
1st Image Caption |
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. |
2nd Image Caption |
Flow cytometric analysis of WiDr cells using FGFR4 Antibody (bottom histogram) compared to a negative control(top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
3rd Image Caption |
Fluorescent confocal image of HeLa cells stained with FGFR4 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with FGFR4 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the FGFR4 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000160867). |
4th Image Caption |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.