Eotaxin-2 Antibody (biotin)

Primary Antibodies, Antibody Pairs

Eotaxin-2 antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.

Eotaxin is a 74 amino acid, eosinophil chemotactic CC chemokine originally found in bronchoalveolar lavage fluid from allergic inflammatory subjects. It is involved in regulating the recruitment and activation of inflammatory leukocytes, particularly eosinophils. Eotaxin binds and activates the CCR3 chemokine receptor, and may play a fundamental role in the development of allergic responses.

None

Anti-mEotaxin-2 specific antibody was purified by affinity chromatography and then biotinylated.

ELISA:
Sandwich: To detect mEotaxin-2 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.25 - 1.0 μ g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine Eotaxin-2 (XP-5125) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEotaxin-2.
Western Blot:
To detect mEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEotaxin-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Polyclonal

Mus musculus

Centrifuge vial prior to opening.