IL-10 Antibody

ELISA, WB, Neutralization Assay

IL-10 antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.

Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.

None

Anti-mIL-10 specific antibody was purified by affinity chromatography employing immobilized mIL-10 matrix.

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (2.5 ng/mL), a concentration of 0.012- 0.014 μ g/mL of this antibody is required.
ELISA:
To detect mIL-10 by direct ELISA (using 100 μ L/well antibody solution) a concentration of at least 0.5 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-10.
Sandwich
To detect mIL-10 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IL-10 (XP-5162Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-10.
Western Blot:
To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Polyclonal

Mus musculus

Centrifuge vial prior to opening.