IL-1a Antibody

Primary Antibodies, Functional Antibodies, Antibody Pairs

Room Temp

Immunology, Chemokines & Cytokines, Antibody Pairs, Functional Assays

None

Anti-Rat IL-1alpha specific antibody was purified by affinity chromatography employing immobilized Rat IL-1alpha matrix.

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-1α (50pg/mL), a concentration of 0.011-0.017 μ g/mL of this antibody is required.
ELISA:
To detect Rat IL-1α by direct ELISA (using 100 μ L/well antibody solution) a concentration of at least 0.5 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat IL-1α .
Sandwich:
To detect Rat IL-1α by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IL-1α (XP-5176Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1α .
Western Blot:
To detect Rat IL-1α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-1α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Polyclonal

Rattus norvegicustus norvegicus

Centrifuge vial prior to opening.