IL-6 Antibody

ELISA, WB, Neutralization Assay

IL-6 antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.

IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL-6 appears to interact with IL2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass.

None

Anti-mIL-6 specific antibody was purified by affinity chromatography employing immobilized mIL-6 matrix.

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-6 (0.1 ng/mL), a concentration of 0.012 -0.014 μ g/mL of this antibody is required.
ELISA:
To detect mIL-6 by direct ELISA (using 100 μ L/well antibody solution) a concentration of at least 0.5 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-6.
Sandwich:
To detect mIL-6 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IL-6 (XP-5194Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-6.
Western Blot:
To detect mIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Polyclonal

Mus musculus

Centrifuge vial prior to opening.