Hersteller |
ProSci
|
Kategorie |
|
Typ |
Antibody Primary |
Specific against |
Human |
Format |
Lyophilized |
Applikationen |
WB, ELISA |
Menge |
0.1 mg |
Host |
Rabbit |
ArtNr |
PRS-XP-5257-0.1mg |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Chemokines & Cytokines, Antibody Pairs, Functional Assays |
Immunogen |
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hRANTES (human RANTES). |
Applications |
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hRANTES (100 ng/mL), a concentration of 5.0 - 7.0 μ g/mL of this antibody is required.
ELISA:
To detect hRANTES by direct ELISA (using 100 μ L/well antibody solution) a concentration of at least 0.5 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hRANTES.
Sandwich:
To detect hRANTES by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human RANTES (XP-5257Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hRANTES.
Western Blot:
To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Purification |
Anti-hRANTES specific antibody was purified by affinity chromatography employing immobilized hRANTES matrix. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Storage Conditions |
RANTES antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CCL5 |
Accession # |
P13501 |
Protein Gi # |
6175077 |
Ncbi Gene Id # |
6352 |
User Note |
Centrifuge vial prior to opening. |
Ncbi Official Symbol |
CCL5 |
Ncbi Official Full Name |
chemokine (C-C motif) ligand 5 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P13501 |
Background |
RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family. |
1st Image Caption |
To detect hRANTES by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human RANTES (XP-5257Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hRANTES. |
2nd Image Caption |
To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
3rd Image Caption |
To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
4th Image Caption |
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.0 ug/ml with an overnight incubation at 4˚ C. An HRP-labeled polymer detection system was used with an AEC chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary. |
5th Image Caption |
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.0 ug/ml with an overnight incubation at 4˚ C. An HRP-labeled polymer detection system was used with an AEC chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.