TWEAK Antibody (biotin)

Primary Antibodies, Antibody Pairs

TWEAK antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.

TWEAK is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family.

None

Anti-hTWEAK specific antibody was purified by affinity chromatography and then biotinylated.

ELISA:
Sandwich:
To detect hTWEAK by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.25 - 1.0 μ g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human TWEAK (XP-5290) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTWEAK.
Western Blot:
To detect hTWEAK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTWEAK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Polyclonal

Homo sapiens

Centrifuge vial prior to opening.