VEGF Antibody

Room Temp

Signal Transduction, Growth Factor, Antibody Pairs

None

Anti-hVEGF specific antibody was purified by affinity chromatography employing immobilized hVEGF matrix.

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hVEGF (10 ng/mL), a concentration of 0.05 - 0.1 μ g/mL of this antibody is required.
ELISA:
To detect hVEGF by direct ELISA (using 100 μ L/well antibody solution) a concentration of at least 0.5 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hVEGF.
Sandwich:
To detect hVEGF by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human VEGF (XP-5291Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF.
Western Blot:
To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Polyclonal

Homo sapiens

Centrifuge vial prior to opening.