NAP-2 Antibody [G6_5C7.3]

ELISA:

In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human NAP-2 when used with our biotinylated antigen affinity purified anti-human NAP-2 (XP-5244Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.

Western blot:

To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.

NAP-2 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

NAP2 is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

This IgG1/K antibody was purified from a Protein A chromatography column.

Monoclonal

Homo sapiens

Centrifuge vial prior to opening.