Anti-Argininosuccinate synthetase 1 antibody (Internal) (STJ71128)

Pep-ELISA/WB/IF/FC/IHC

Store at-20°C on receipt and minimise freeze-thaw cycles.

0.5 mg/mL

Polyclonal

Peptide ELISA: antibody detection limit dilution 1:64000.
WB: Approx 45kDa band observed in lysates of cell lines A431 and NIH3T3 and approx.48kDa in Human and Rat Kidney and in Mouse Liver lysates (calculated MW of 46.5kDa according to Human NP_000041.2 and Rat NP_037289.1, and 46.6kDa according to Mouse NP_031520.1 ). Recommended concentration: 0.03-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Kidney shows textured cytoplasm staining in PCT. Recommended concentration: 2-4µg/ml. Paraffin embedded Human Liver. Recommended concentration: 2.5µg/ml.
IF: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

FC:Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Internal