Anti-CAMK2A antibody (Internal) (STJ71922)

Pep-ELISA/WB/IF/FC

Store at-20°C on receipt and minimise freeze-thaw cycles.

0.5 mg/mL

Polyclonal

Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 48-50+60kDa bands observed in Human Cerebral Cortex and Mouse and Rat Brain lysates, corresponding to CAMK2alpha and CAMK2beta respectively and approx. 60kDa in Human Cerebellum lysates (calculated MW of 55.3kDa according to Human NP_057065.2 and 54.1kDa according to Mouse NP_001390241.1 and Rat NP_037052.1 subunit Alpha and 60.4kDa according to Human NP_001280099.1, Mouse NP_031621.3 and Rat NP_068507.2 subunit beta). These molecular weights are routinely observed by other sources, and were successfully blocked by incubation with the immunizing peptide. Recommended concentration 0.1-1ug/ul. Primary incubation 1 hour at room temperature.
IF: Strong expression of the protein seen in the cytoplasm of Neuro2a cells. Recommended concentration: 10µg/ml.

FC:Flow cytometric analysis of Neuro2a cells. Recommended concentration: 10ug/ml.

Internal