Anti-Vimentin antibody (C-Term) (STJ73113)

Pep-ELISA/WB/FC/IF/IHC

Store at-20°C on receipt and minimise freeze-thaw cycles.

0.5 mg/mL

Polyclonal

Peptide ELISA: antibody detection limit dilution 1:4000.
WB: Approx 55kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60kDa band in Rat Ovary lysates (calculated MW of 53.7kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1). Recommended concentration: 0.1-2µg/ml. This antibody has been successfully used in WB on Human, PMID: 35769261.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.
FC:Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.IF: Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. Recommended concentration: 5µg/ml. This antibody has been successfully used in IF on Human: https://doi.org/10.1101/2021.05.04.442648, and PMID: 35487944.

C-Term