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Rabbit anti-p47 phox Antibody

Rabbit anti-p47 phox Antibody

Hersteller	YL Biotech
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Polyclonal
Specific against	Human, Mouse, Monkey, Pig
Applikationen	WB, IF, IHC, IC
Menge	50ul
Host	Rabbit
ArtNr	YLD5837-50ul
eClass 6.1	32160702
eClass 9.0	32160702
Lieferbar
Background	Rabbit polyclonal antibody to p47 phox
Concentration	1mg/mL
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human p47 phox. The exact sequence is proprietary.
WB description	Western blot analysis of p47 phox expression in Jurkat (A), THP1 (B) whole cell lysates.
IHC description	Immunohistochemical analysis of p47 phox staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description	Immunofluorescent analysis of p47 phox staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Purification_Method	The antibody was purified by immunogen affinity chromatography.
Clonality	Polyclonal
Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution	WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)
Storage	Store at -20℃. Avoid repeated freeze / thaw cycles.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 50ul

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Lieferung vsl. bis 13.06.2024

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