BRD3 Inhibitor Screening, BioAssay™ Kit (BD1, BRD3, RING3L, Bromodomain)

Kits and Assays / Kits-Molecular Biology, BioAssay™

-20°C/-70°C

The BRD3 (BD1) Inhibitor Screening BioAssay™ Kit is designed to measure the inhibition of BRD3 bromodomain 1 (BD1) from binding to its substrate. The key to the BRD3 (BD1) Inhibitor Screening BioAssay™ Kit is the highly specific binding of the BRD3 bromodomain 1 to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRD3 bromodomain 1 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

Applications:
Suitable for use in study of bromodomain binding assays, screening inhibitors and selectivity profiling. Other applications not tested.

Kit Components:
BRD3 (29-145), BD1, GST-tag, 1x20ug
BET Bromodomain Ligand, 1x400ul
Non-acetylated Ligand 1, 1x200ul
3x BRD Homogeneous Assay Buffer 1, 1x4ml
3x BRD Homogeneous Detection Buffer 1, 1x3ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.