Vergleich

Anti-NFH (Neurofilament Heavy Chain) Antibody

ArtNr NFH
Hersteller Aves Labs
Menge 200 uL
Kategorie
Typ Antibody Polyclonal
Format Liquid
Applikationen WB, IHC, ICC
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Chicken
Isotype IgY
Konjugat/Tag Unconjugated
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Primary Antibody
Manufacturer - Category
Cell Markers, Neuronal Markers, Axonal Markers, Neurodegenerative Disease
Manufacturer - Targets
Neurofilament Heavy Chain (NFH)
Country of Origin
United States
Storage Conditions
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Molecular Weight
200-220 kDa
Concentration
1 mg/mL
Buffer
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative
Quality Control
Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:50000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Description
NFH protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion” (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFH protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. These antibodies have been found to cross-react with NFH protein from a variety of species, including human, mouse and rat.
Manufacturer - Statement
We produce our Anti-Neurofilament Heavy Chain (NFH) chicken polyclonal primary antibody. It is great in IHC, ICC, WB and is IgY fraction.
Target Description
Human Neurofilament Heavy Chain (NFH) is a 115, 378 dalton protein (1072 amino acids) that forms part of the neuronal cytoskeleton. NFH is physically associated with 10 nm intermediate filaments and is said to be one of the "neurofilament triplet" proteins, although it probably serves more to stabilize the cytoskeleton rather than as an integral structural component. NFH immunoreactivity is found in neuronal somata, dendrites and axons.
Antibody Registry ID (RRID)
AB_2313552
Clonality
Polyclonal
Dilution Range ICC
1:2000-1:5000
Dilution Range IHC
1:2000-1:5000
Dilution Range Western Blot
1:5000-1:10000
Immunogen
Native NF-H purified from bovine spinal cord
Immunogen Species
Cow
Manufacturer - Form
IgY Fraction
Expiration
12 months from receipt of product
Usage Statement
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Production Notes
Chickens were immunized with NF-H protein purified from adult bovine brains. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 200 uL
Lieferbar: In stock
lieferbar

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