Anti-Amyloid Precursor Protein (APP), Peptide #3 Antibody

Primary Antibody

Amyloid Precursor Protein (APP), Peptide #3

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

100 µg/mL

This anti-peptide antibody mixture was analyzed by immunohistochemistry (at a dilution of 1:1000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

This antibody recognizes the peptide sequence QHF QEK VES LEQ EAA NER AA, which corresponds to residues #433-452 of the human APP gene product. This peptide sequence is 100% conserved between human, mouse and rat homologs of APP, and the antibodies against this sequence cross-react with all three species. The antibody preparation is at a concentration of 100 µg/mL (based on absorbance at 280 nm).

Amyloid Precursor Protein (APP, UniProt Accession Number P05067) is a 770 amino acid, single-pass transmembrane protein whose beta-amyloid proteolytic fragment can form neurotoxic extracellular accumulations in human cerebral cortex, and is widely believed to be the cause of Alzheimer's dementia. However, the normal function of APP itself is still obscure, as are potential functions of various proteolytic fragments that have been observed in human brain [Muller, U.C., Deller, T., Korte, M. (2017) Nature Reviews Neuroscience 18: 281-298)]. To better understand these functions, we have made a set of five anti-peptide antibody reagents against APP. One of the peptide sequences used is within the beta-amyloid fragment and recognizes the extracellular amyloid plaques observed in Alzheimer's patients' brains (see Cat.# ABN). Three other peptide sequences used, however, are within the extracellular domain of APP outside of this domain, and are useful for identifying the APP protein itself, and various proteolytic fragments, rather than the beta-amyloid peptide and plaques.

Polyclonal

1:500-1:1000

Human

12 months from receipt of product

Chickens were immunized with a keyhole limpet hemocyanin (KLH) conjugate of the synthetic peptide QHF QEK VES LEQ EAA NER QQ, which corresponds to residues #433-452 of the human gene product (NP_000475.1). This sequence is also conserved in the mouse and rat APP gene products, as well. After repeated injections, immune eggs were collected from the hens, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified against the cognate peptide using an agarose column, the concentration of the eluates adjusted to 100 µg/mL, and the preparation was filter-sterilized.