Anti-Synaptotagmin-1 Antibody

Primary Antibody

Synaptotagmin-1

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

400 µg/mL

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Regular vials contain 1000 ul of this antibody mixture, Sampler vials contain 200 µ, l of this mixture.

We produce our Anti-Synaptotagmin-1 chicken polyclonal primary antibody. It is great in IHC, ICC, WB and is affinity-purified IgY.

AB_2313562

1:1000-1:2000

1:1000-1:2000

Human, Mouse, Rat

12 months from receipt of product

Chickens were immunized with four synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Synaptotagmin-1 gene product, but are shared between the human (NP_005630, NCBI) and mouse (NP_033332, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, and the concentrations of the eluates adjusted to 400 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.