MAPKAPK2 Antibody - middle region

Polyclonal Antibody

Wet Ice

Western Blot: Approx 60kDa band observed in Mouse Embryonic Fibroblasts (MEF) lysates, not present in the knockout MEFs and reappearing as a 45kDa band upon reintroduction of MK2 gene expression through viral transduction (calculated MW of 44.0kDa according to Mouse NP_032577.1). The observed molecular weights correspond to earlier findings in literature with different antibodies (Menon et al, Cell Motil Cytoskeleton. 2009 Dec; 66(12):1041-7. PMID: 19743408). There is no cross-reactivity to MK5.
Peptide ELISA: antibody detection limit dilution 1:128000.
Immunoprecipitation: MK2/MK3 double knockout MEFs retrovirally transduced wiith MK2 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11929 or EB11930. Western blots of the IP were labelled with rabbit anti-MK2 (CST). An approx 45kDa of MK2 is only precipitated from lysates of those KO MEFs that have been rescued by the MK2 expression construct as described in PMID: 19743408. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

MAP kinase-activated protein kinase 2|MAPK activated protein kinase 2

Aliquot and store at -20C. Minimize freezing and thawing.

Polyclonal

MAPKAPK2

middle region (near C-terminal region)