Anti-NMDA Receptor, NR1 Subunit, C1 Splice Variant Insert

Polyclonal Antibody

Wet Ice

The antibody has been directly tested for reactivity in Western blots in rat and mouse tissues. It is anticipated that the antibody will also work with bovine, canine, human, and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Western blot of 10 ug of HEK 293 cells expressing: Lane 1 - HEK cells without NR1 expression (Mock); Lane 2 - NR1 subunit containing only the C2 Insert; Lane 3 - NR1 subunit containing the C1 and C2' Insert; Lane 4 - NR1 subunit containing the N1 and C2' Insert showing specific immunolabeling of the ~120k NR1 subunit of the NMDA receptor containing the C1 splice variant insert.

Lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody should be aliquoted and

The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory and neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.

Glutamate receptor ionotropic, NMDA 1

Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Cow; Dog; Human; Mouse; Rat