Anti-Neurofilament M (NF-M)

Polyclonal Antibody

Wet Ice

The antibody has been directly tested for reactivity in chicken, human, mouse and rat tissues.
Immunostaining of cultured rat neurons and glia showing labeling of NF-M in red.
Western blot of rat cortex lysate showing specific immunolabeling of the ~ 145k NF-M protein.

Liquid containing 10 mM sodium azide.

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (2).

Neurofilament medium polypeptide

Total IgY fraction.

Human; Mouse; Rat