Anti-NMDA Receptor, NR1 Subunit, C2 Splice Variant Insert

Polyclonal Antibody

Wet Ice

The antibody has been directly tested for reactivity in Western blots in rat and mouse tissues. It is anticipated that the antibody will also work with human tissues based on the fact that this species has 100% homology with the amino acid sequence used as antigen.
Western blot of 10 ug of HEK 293 cells showing specific immunolabeling of the ~120k NR1 subunit of the NMDA receptor containing the C2 splice variant insert. Lane 1 - HEK cells without NR1 expression; Lane 2 - NR1 subunit containing only the C2 Insert; Lane 3 - NR1 subunit containing the C1 and C2' insert; Lane 4 - NR1 subunit containing the N1 and C2' insert.

Lyophilized from 5 mM ammonium bicarbonate. Adequate amount of material to conduct 10-mini Western Blots.

The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1 subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1 subunit generates up to eight NR1 subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.

Glutamate receptor ionotropic, NMDA 1

Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Human; Mouse; Rat