Anti-Vimentin

Polyclonal Antibody

Wet Ice

The antibody has been directly tested for reactivity in human, mouse and rat. It is expected that it will work on other mammalian tissues.
Western blot of NIH 3T3 cells showing specific immunolabeling of the ~50k Vimentin protein.
Mixed neuron/glial cultures stained with anti-Vimentin (green) and rabbit anti-GFAP antibody (catalog #OAPC00114) (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.

Liquid. Contains 10 mM sodium azide.

Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008).

Vimentin

Total IgY fraction.

Human; Mouse; Rat