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GluR1 Antibody (Phospho-Ser845)

GluR1 Antibody (Phospho-Ser845)

ArtNr	OAPC00169
Hersteller	AVIVA Systems Biology
Menge	150 ul
Kategorie	Neuroscience Molecular Targets for Neuroscience Neural Signaling Antibodies Immunohistochemistry Western Blot Primary Antibodies
Typ	Antibody Polyclonal
Format	Liquid
Applikationen	WB, IHC
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Citations	Banke TG, Bowie D, Lee HK, Huganir RL, Schousboe A, Traynelis SF (2000) Control of GluR1 AMPA receptor function by cAMP-dependent protein kinase. J Neurosci 20:89-102. Esteban JA, Shi SH, Wilson C, Nuriya M, Huganir RL, Malinow R (2003) PKA phosphorylation of AMPA receptor subunits controls synaptic trafficking underlying plasticity. Nature Neurosci 6:136-143. Hollmann M, Heinemann S (1994) Cloned glutamate receptors. Annu Rev Neurosci 17:31-108. KeinÃƒÂ¤nen K, Wisden W, Sommer B, Werner P, Herb A, Verdoorn TA, Sakmann B, Seeburg PH (1990) A family of AMPA-selective glutamate receptors. Science 249:556-560. Lee HK, Takamiya K, Han JS, Man HY, Kim CH, Rumbaugh G, Yu S, Ding L, He C, Petralia RS, Wenthold RJ, Gallagher M, Huganir RL (2003) Phosphorylation of the AMPA receptor GluR1 subunit is required for synaptic plasticity and retention of spatial memory. Cell 112(5):631-643. Roche KW, O'Brien RJ, Mammen AL, Bernhardt J, Huganir RL (1996) Characterization of multiple phosphorylation sites on the AMPA receptor GluR1 subunit. Neuron 16:1179-1188. Sun X, Milovanovic M, Zhao Y, Wolf ME. (2008) Acute and chronic dopamine receptor stimulation modulates AMPA receptor trafficking in nucleus accumbens neurons cocultured with prefrontal cortex neurons. J Neurosci. Apr 16;28(16):4216-30. .Robert M. Sears, Rong-Jian Liu, Nandakumar S. Narayanan, Ruth Sharf, Mark F. Yeckel, Mark Laubach, George K. Aghajanian, and Ralph J. DiLeone (2010) Regulation of Nucleus Accumbens Activity by the Hypothalamic Neuropeptide Melanin-Concentrating Hormone J. Neurosci., 30: 8263 - 8273. Heike Rebholz, Akinori Nishi, Sabine Liebscher, Angus C. Nairn, Marc Flajolet, and Paul Greengard (2009) CK2 negatively regulates G alpha s signaling PNAS, 106: 14096 - 14101. Emanuela Santini, Myriam Heiman, Paul Greengard, Emmanuel Valjent, and Gilberto Fisone (2009) Inhibition of mTOR Signaling in Parkinson's Disease Prevents L-DOPA-Induced Dyskinesia Sci. Signal., Jul 2009; 2: ra36. Zhaoqing Zheng and Joyce Keifer (2009) PKA Has a Critical Role in Synaptic Delivery of GluR1- and GluR4- Containing AMPARs During Initial Stages of Acquisition of In Vitro Classical ConditioningJ Neurophysiol, 101: 2539 - 2549. Douglas A. Meyer, Edmond Richer, Stanley A. Benkovic, Kanehiro Hayashi, Janice W. Kansy, Carly F. Hale, Lily Y. Moy, Yong Kim, James P. O'Callaghan, Li-Huei Tsai, Paul Greengard, Angus C. Nairn, Christopher W. Cowan, Diane B. Miller, Pietro Antich, and James A. Bibb (2008) Striatal dysregulation of Cdk5 alters locomotor responses to cocaine, motor learning, and dendritic morphology. PNAS, 105: 18561 - 18566. Kurtis D. Davies, Susan M. Goebel-Goody, Steven J. Coultrap, and Michael D. Browning (2008) Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not alpha -Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization J. Biol. Chem., 283: 33138 - 33146. Akinori Nishi, Mahomi Kuroiwa, Diane B. Miller, James P. O'Callaghan, Helen S. Bateup, Takahide Shuto, Naoki Sotogaku, Takaichi Fukuda, Nathaniel Heintz, Paul Greengard, and Gretchen L. Snyder (2008) Distinct Roles of PDE4 and PDE10A in the Regulation of cAMP/PKA Signaling in the Striatum J. Neurosci., 28: 10460 - 10471 Xiu Sun, Michael Milovanovic, Yun Zhao, and Marina E. Wolf (2008) Acute and Chronic Dopamine Receptor Stimulation Modulates AMPA Receptor Trafficking in Nucleus Accumbens Neurons Cocultured with Prefrontal Cortex Neurons. J. Neurosci., 28: 4216 - 4230.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GluA1,gluR-A
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	Polyclonal Antibody
Manufacturer - Category	Root Catalog/Products/Polyclonal Antibodies, Root Catalog/Products/Polyclonal Antibodies/Phospho Specific, Root Catalog/Products/Primary Antibodies
Shipping Temperature	Wet Ice
Molecular Weight	~100kDa
Manufacturer - Application Additional Information	The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with human, mouse and non-human primate based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser845 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda -Ptase). The blot is identical to the control except that it was incubated in lambda -Ptase (1200 units for 30 min) before being exposed to the GluR1 Ser845 antibody. The immunolabeling is completely eliminated by treatment with lambda -Ptase.
Gene symbol	GRIA1
Product format	Liquid. 150 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Reconstitution and storage	For long term storage -20C is recommended. Stable at -20C for at least 1 year.
Description of target	The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha -amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (KeinÃƒÂ¤nen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Lee at al., 2003; Esteban at al., 2003).
Protein accession_num	NP_113796.1
Protein name	Glutamate receptor 1
Clonality	Polyclonal
Purification	Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen	Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser845 of GluR1.
Homology	Human; Mouse; Rat
Manufacturer - Specificity	Specific for the ~100k GluR1 protein phosphorylated at Ser845 in Western blots of rat brain extracts. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is s completely eliminated by treatment with lambda -Ptase.

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Menge: 150 ul

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Lieferung vsl. bis 29.01.2026

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