MMP3 Antibody (OASA03130)

Immunohistology - Paraffin: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. Western Blotting: OASA03130 detects a band of approximately 50kDa in cell lysates.

MMP3

Store at +4C or at -20C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

The immunogen for anti-MMP3 antibody: ovalbumin conjugated synthetic peptide corresponding to a region within the C-terminus of human MMP-3.

Mouse anti Human MMP-3 antibody, clone 1B4 recognizes human matrix metalloproteinase 3, also known as MMP-3, stromelysin-1 or transin-1. MMP-3 in its pro-form is a 477 amino acid ca.54kDa zinc dependent endopeptidase processed to a 378 amino acid, ca.45kDa peptide in the mature form containing 4 hemopexin-like repeats.

MMPs are also involved in cell proliferation, migration, differentiation and apoptosis (Jana et al. 2012). Most MMP's are synthesised as inactive zymogens and a propeptide region must be cleaved off before the enzyme becomes active. Their expression is increased dramatically in a variety of cancer types, where it indicates invasive disease and a poor prognosis (Holmberg et al. 2013). MMP-3 can degrade proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen (Wilhelm et al. 1993). It can activate other MMP's and may be involved in the repair processes of the heart after myocardial infarction (Wang et al. 2011).

Mouse anti human MMP-3 antibody, clone 1B4 has been used successfully for the identification of MMP-3 in gastric cancer by both Western blotting and immunohistochemistry (Murray et al. 1998)