Anti-GABA Alpha Receptor, alpha 3 -Subunit

Polyclonal Antibody

Wet Ice

DB: 1:1000WB: 1:1000IHC: 1:100

Liquid 10mM HEPES (pH 7.5), 150mM NaCl, 100 ug/ml BSA and 50% glycerol. Adequate amount of material to conduct 10 mini Western blots.

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alpha s, four beta s and four gamma s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha - and beta -subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma -subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha -subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Polyclonal

Fusion protein from N-terminal region of the alpha 3-subunit of rat GABAA receptor.

Specific for the ~51k alpha 3-subunit of the GABAA-R receptor in Western blots of rat and mouse brain lysate.