grpE Antibody

Root Catalog/Products/Polyclonal Antibodies, Root Catalog/Products/Primary Antibodies

Western Blot (1:000, colorimetric)
Optimal conditions must be determined individually for each application.

nucleotide exchange factor GrpE

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.

WP_001296310.1; YP_670504.1

Polyclonal

Recognizes bacteria GrpE. Detects a band of ~24kDa by Western blot.