Anti-6X HIS EPITOPE TAG Antibody

Root Catalog/Products/Monoclonal Antibodies, Root Catalog/Products/Primary Antibodies

ELISA: 1:20, 000 - 1:100, 000 WB: 1:2, 000 - 1:10, 000 IHC: User Optimized OTHER: FLOW CYTOMETRY: 1:2, 000 - 1:10, 000

Store vial at -20 C or below prior to opening. This vial contains a relatively low volume of reagent (25 uL). To minimize loss of volume dilute 1:10 by adding 225 uL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20 C or below after dilution. Avoid cycles of freezing and thawing. Expiration date is three (3) months from date of opening.

This His antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.