CCL4/MIP-1 beta ELISA Kit (Rat)

ELISA Kit

Wet Ice

chemokine (C-C motif) ligand 4

Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in mouse is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. Based on functional analysis of MIP-1β, one or more additional β chemokine receptors that transduce MIP-1β signals must also exist. MIP-1α and MIP-1β have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many α as well as β chemokines (11, 14).

NP_446310.1

Expression system for standard: E.coli; Immunogen sequence: A24-N92

Assay Methodology: Quantitative Sandwich Immunoassay

Aviva's rat CCL4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL4 has been precoated onto 96-well plates. Standards(E.Coli, A24-N92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat CCL4 amount of sample captured in plate.