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OSM ELISA Kit (Mouse)

OSM ELISA Kit (Mouse)

ArtNr

OKBB00734

Hersteller

AVIVA Systems Biology

Menge

96 wells/kit, with removable strips.

Kategorie

Typ

Elisa-Kit

Applikationen

ELISA

Specific against

Mouse (Murine, Mus musculus)

Konjugat/Tag

Biotin

Sensitivity

<10 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

MGC20461,ONCM_HUMAN,Oncostatin M precursor,Oncostatin-M,OncostatinM,OSM,OTTMUSP00000005249,RP23-453B22.1

Versandbedingung

Gekühlt

Lieferbar

Manufacturer - Type

ELISA Kit

Manufacturer - Category

Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits

Shipping Temperature

Wet Ice

Gene symbol

OSM

Gene Fullname

oncostatin M

Reconstitution and storage

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Description of target

OSM(Oncostatin M) is a protein that in humans is encoded by the OSM gene. OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. The OSM gene is mapped on 22q12.2. Of these cytokines it most closely resembles leukemia inhibitory factor(LIF) in both structure and function. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR.

Nucleotide accession_num

NM_001013365.2

Protein accession_num

NP_001013383.1

Protein name

Oncostatin-M

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A24-R206

Manufacturer - Specificity

Natural and recombinant mouse Oncostatin M

Kit duration

~ 3 Hours

Kit principle

Aviva's mouse Oncostatin M ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Oncostatin M has been precoated onto 96-well plates. Standards(E.coli, A24-R206) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Oncostatin M is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Oncostatin M amount of sample captured in plate.

Kit reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample
Sample Type	cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKBB00734.pdf

OKBB00734-datasheet.pdf

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Einfache Bestellung Periodische Bestellung

Menge: 96 wells/kit, with removable strips.

lieferbar

Lieferung vsl. bis 27.11.2025

Express-Lieferung: 10 Tage sparen

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