TGFA ELISA Kit (Horse)

ELISA Kit

Wet Ice

Protransforming growth factor alpha

Transforming growth factor alpha(TGF-alpha) is upregulated in some horse equine cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene(TGFA) maps to horse equine chromosome 2 close to the breakpoint of the t(2; 8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic horse equine TGF-alpha showed similar immunoreactivity when compared with horse equine TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.

NP_001093161.1

Expression system for standard: E.coli; Immunogen sequence: V40-A89

Aviva's Equine TGF alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF alpha has been precoated onto 96-well plates. Standards (E.coli, V40-A89) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Equine TGF alpha amount of sample captured in plate.