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CXCL5 ELISA Kit (Rat)

ArtNr OKBB00826
Hersteller AVIVA Systems Biology
Menge 96 wells/kit, with removable strips.
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Rat (Rattus norvegicus)
Konjugat/Tag Biotin
Sensitivity <1 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias AMCFII,C-X-C motif chemokine 5,chemokine (C-X-C motif) ligand 5,Cxcl5,CXCL5_HUMAN,ENA-78 (8-78),ENA-78(1-78),ENA-78(9-78),ENA78,Epithelial derived neutrophil activating peptide 78,Epithelial derived neutrophil activating protein 78,Epithelial-derived neutrophil-activating protein 78,GCP2,Lipopolysaccharide-induced CXC chemokine,LIX,Neutrophil activating peptide ENA 78,Neutrophil-activating peptide ENA-78,neutrophil-activating protein 78,SCYB5,Scyb6,Small inducible cytokine B5,small inducible cytokine subfamily B (Cys-X-Cys),member 5 (epithelial-derived neutrophil-activating peptide 78),Small inducible cytokine subfamily B member 5,small inducible cytokine subfamily B,member 5,Small-inducible cytokine B5
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits
Shipping Temperature
Wet Ice
Gene symbol
CXCL5
Gene Fullname
chemokine (C-X-C motif) ligand 5
Reconstitution and storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Description of target
C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78(ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.
Nucleotide accession_num
NM_022214.1
Protein accession_num
NP_071550.1
Protein name
C-X-C motif chemokine 5
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A38-Q130
Manufacturer - Specificity
Natural and recombinant rat CXCL5
Kit duration
~ 3 Hours
Kit principle
Aviva's rat CXCL5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL5 has been precoated onto 96-well plates. Standards(E.coli, A38-Q130) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat CXCL5 amount of sample captured in plate.
Kit reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample
Sample Type
cell culture supernates, serum and plasma (heparin, EDTA, citrate)

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Menge: 96 wells/kit, with removable strips.
Lieferbar: In stock
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