THBS1 ELISA Kit (Monkey)

ELISA Kit

Wet Ice

Thrombospondin-1

Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to monkey primate 15q15 and the cognate gene to monkey primate chromosome 2(region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.

NP_003237.2

Expression system for standard: NS0; Immunogen sequence: N19-P1170

Aviva's Monkey THBS1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for THBS1 has been precoated onto 96-well plates. Standards(NS0, N19-P1170) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for THBS1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Monkey THBS1 amount of sample captured in plate.