EDN1 ELISA Kit (Horse)

ELISA Kit

Wet Ice

Endothelin-1

The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of horse equine endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure(hypertension) and heart disease.

NP_001161791.1

Expression system for standard: horse equine peptide; Immunogen sequence: C53-W73

Aviva's Equine EDN1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for EDN1 has been precoated onto 96-well plates. Standards(human peptide, C53-W73) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for EDN1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Equine EDN1 amount of sample captured in plate.