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A2M ELISA Kit (Human)

A2M ELISA Kit (Human)

ArtNr

OKBB00901

Hersteller

AVIVA Systems Biology

Menge

96 wells/kit, with removable strips.

Kategorie

Typ

Elisa-Kit

Applikationen

ELISA

Specific against

Human (Homo sapiens)

Konjugat/Tag

Biotin

Sensitivity

<20 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5,A2m,A2MD,A2MG_HUMAN,Alpha-2-M,Alpha-2-macroglobulin,C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5,CPAMD5,FWP007,Pregnancy zone protein,S863-7

Versandbedingung

Gekühlt

Lieferbar

Manufacturer - Type

ELISA Kit

Manufacturer - Category

Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits

Shipping Temperature

Wet Ice

Gene symbol

A2M

Gene Fullname

alpha-2-macroglobulin

Reconstitution and storage

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Description of target

Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease(AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates(activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Nucleotide accession_num

NM_000014.5

Protein accession_num

NP_000005.2

Protein name

Alpha-2-macroglobulin

Immunogen

Expression system for standard: from human plasma

Manufacturer - Specificity

Natural human A2M

Kit duration

~ 3 Hours

Kit principle

Aviva's human A2M ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for A2M has been precoated onto 96-well plates. Standards(from human plasma) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for A2M is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human A2M amount of sample captured in plate.

Kit reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample
Sample Type	cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKBB00901.pdf

OKBB00901-datasheet.pdf

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Einfache Bestellung Periodische Bestellung

Menge: 96 wells/kit, with removable strips.

lieferbar

Lieferung vsl. bis 08.01.2026

Express-Lieferung: 1 Tage sparen

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