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MDK ELISA Kit (Human)

MDK ELISA Kit (Human)

ArtNr

OKBB00922

Hersteller

AVIVA Systems Biology

Menge

96 wells/kit, with removable strips.

Kategorie

Typ

Elisa-Kit

Applikationen

ELISA

Specific against

Human (Homo sapiens)

Konjugat/Tag

Biotin

Sensitivity

<10 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Amphiregulin-associated protein,ARAP,Mdk,Midgestation and kidney protein,Midkine,MK,MK_HUMAN,Neurite outgrowth-promoting factor 2,Neurite outgrowth-promoting protein

Versandbedingung

Gekühlt

Lieferbar

Manufacturer - Type

ELISA Kit

Manufacturer - Category

Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits

Shipping Temperature

Wet Ice

Gene symbol

MDK

Gene Fullname

midkine (neurite growth-promoting factor 2)

Reconstitution and storage

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Description of target

Midkine(MK or MDK) also known as neurite growth-promoting factor 2(NEGF2) is a protein that in humans is encoded by the MDK gene. Midkine is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin(NEGF1, 46% homologous with MK). It is a nonglycosylated protein, composed of two domains held by disulfide bridges. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. MK is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. A molecular complex containing receptor-type tyrosine phosphatase zeta(PTPÎ¶), low density lipoprotein receptor-related protein(LRP1), anaplastic leukemia kinase(ALK) and syndecans is considered to be its receptor.

Nucleotide accession_num

NM_001012333.2

Protein accession_num

NP_001012333.1

Protein name

Midkine

Immunogen

Expression system for standard: E.coli; Immunogen sequence: K23-D143

Manufacturer - Specificity

Natural and recombinant human Midkine

Kit duration

~ 3 Hours

Kit principle

Aviva's human Midkine ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Midkine has been precoated onto 96-well plates. Standards(E.coli, K23-D143) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Midkine is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Midkine amount of sample captured in plate.

Kit reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample
Sample Type	cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKBB00922.pdf

OKBB00922-datasheet.pdf

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Einfache Bestellung Periodische Bestellung

Menge: 96 wells/kit, with removable strips.

lieferbar

Lieferung vsl. bis 08.01.2026

Express-Lieferung: 7 Tage sparen

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