Vergleich

FOLR1 ELISA Kit (Human)

ArtNr OKBB00925
Hersteller AVIVA Systems Biology
Menge 96 wells/kit, with removable strips.
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
Konjugat/Tag Biotin
Sensitivity <10 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Adult folate binding protein,Adult folate-binding protein,FBP,Folate Receptor 1 Adult,Folate receptor 1,Folate Receptor 1 Precursor,Folate receptor adult,Folate receptor alpha,Folate receptor,FOLR,FOLR1,FOLR1_HUMAN,FR alpha,FR-alpha,FRalpha,KB cells FBP,MOV18,Ovarian cancer associated antigen,Ovarian tumor associated antigen,Ovarian tumor associated antigen MOv18,Ovarian tumor-associated antigen MOv18
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits
Shipping Temperature
Wet Ice
Gene symbol
FOLR1
Gene Fullname
folate receptor 1 (adult)
Reconstitution and storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Description of target
Folate receptor 1 adult is a protein that in humans is encoded by the FOLR1 gene. The protein encoded by this gene is a member of the folate receptor(FOLR) family. It is mapped to 11q13.4. FOLR1 expression in Jurkat cells facilitated MBG or EBO entry, and FR-blocking reagents inhibited infection of MBG or EBO. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. The FOLR1 gene encodes the adult folate receptor, or folate-binding protein(FBP), which has a high affinity for folic acid and for several reduced folic acid derivatives, and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is also an important regulator of milk protein synthesis.
Nucleotide accession_num
NM_000802.3
Protein accession_num
NP_000793.1
Protein name
Folate receptor alpha
Immunogen
Expression system for standard: E.coli; Immunogen sequence: R25-S234
Manufacturer - Specificity
Natural and recombinant human FOLR1
Kit duration
~ 3 Hours
Kit principle
Aviva's human FOLR1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FOLR1 has been precoated onto 96-well plates. Standards(E.coli, R25-S234) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FOLR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human FOLR1 amount of sample captured in plate.
Kit reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample
Sample Type
cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine and human milk

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Menge: 96 wells/kit, with removable strips.
Lieferbar: In stock
lieferbar

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