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BMP10 ELISA Kit (Human)

BMP10 ELISA Kit (Human)

ArtNr

OKBB01235

Hersteller

AVIVA Systems Biology

Menge

96 wells/kit, with removable strips.

Kategorie

Typ

Elisa-Kit

Applikationen

ELISA

Specific against

Human (Homo sapiens)

Konjugat/Tag

Biotin

Sensitivity

<10 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Bone morphogenetic protein 10; BMP-10; BMP10

Versandbedingung

Gekühlt

Lieferbar

Manufacturer - Type

ELISA Kit

Manufacturer - Category

Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits

Shipping Temperature

Wet Ice

Gene symbol

BMP10

Gene Fullname

bone morphogenetic protein 10

Reconstitution and storage

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Description of target

Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-Î² superfamily of structurally related signaling proteins. This gene is mapped to 2p13.3. Members of this superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life. BMP-10 plays a crucial role in the development of the embryonic heart by acting to stimulate and maintain cardiomyocyte proliferation. It can signal through various receptor complexes usually containing BMPR-1A, BMPR-1B, ALK1, ALK3, or ALK6. The interaction of BMP-10 with its specific receptors can induce signaling initiated by the phosphorylation of SMAD transcription factors, including SMAD1, SMAD5, or SMAD8, but can also induce SMAD independent processes. BMP-10 is structurally related to BMP-9, and both can inhibit endothelial cell proliferation and migration. Recombinant human BMP-10 is a 24.4 kDa homodimeric disulfide-linked protein consisting of two 108 amino acid subunits, which correspond to amino acid residues 317 to 424 of the full-length BMP-10 precursor.

Nucleotide accession_num

NM_014482.2

Protein accession_num

NP_055297.1

Protein name

Bone morphogenetic protein 10

Immunogen

Expression system for standard: NS0; Immunogen sequence: N314-R421

Manufacturer - Specificity

Natural and recombinant human BMP-10

Kit duration

~ 3 Hours

Kit principle

Aviva's human BMP-10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-10 has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: N314-R421) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.

Kit reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample
Sample Type	cell culture supernates, serum and plasma (heparin, EDTA)

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKBB01235.pdf

OKBB01235-datasheet.pdf

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Einfache Bestellung Periodische Bestellung

Menge: 96 wells/kit, with removable strips.

lieferbar

Lieferung vsl. bis 25.12.2025

Express-Lieferung: 2 Tage sparen

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