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Clec7a ELISA Kit (Mouse)

ArtNr OKBB01305
Hersteller AVIVA Systems Biology
Menge 96 wells/kit, with removable strips.
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Mouse (Murine, Mus musculus)
Konjugat/Tag Biotin
Sensitivity <50 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias C-type lectin domain family 7 member A; Beta-glucan receptor; C-type lectin superfamily member 12; Dendritic cell-associated C-type lectin 1; DC-associated C-type lectin 1; Dectin-1; CD369; Clec7a; Bgr; Clecsf12; Dectin1
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits
Shipping Temperature
Wet Ice
Gene symbol
CLEC7A
Gene Fullname
C-type lectin domain family 7, member a
Reconstitution and storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Description of target
C-type lectin domain family 7 member A or Dectin-1 is a protein that in humans is encoded by the CLEC7A gene. This gene is mapped to 6; 6 F3. CLEC7A is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with a partial immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor for a variety of β-1, 3-linked and β-1, 6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Expression is found on myeloid dendritic cells, monocytes, macrophages and B cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region.
Protein name
C-type lectin domain family 7 member A
Immunogen
Expression system for standard: NS0; Immunogen sequence: G71-L244
Manufacturer - Specificity
Natural and recombinant mouse CLEC7A
Kit duration
~ 3 Hours
Kit principle
Aviva's mouse CLEC7A ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CLEC7A has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: G71-L244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CLEC7A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CLEC7A amount of sample captured in plate.
Kit reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample
Sample Type
cell culture supernates, serum and plasma (heparin, EDTA)

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 wells/kit, with removable strips.
Lieferbar: In stock
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